HIGHLY SELECTIVE HF-LPME-GC-MS FOR COCAINE AND BIOTRANSFORMATION PRODUCTS IN HUMAN HAIR TO MONITOR DRUG ADDICTS

This study aimed to investigate a miniaturized extraction methodology with analysis by GC-MS for simultaneous detection and quantification of cocaine (COC), benzoylecgonine (BZE) cocaethylene (CE) in hair monitor drug addicts. The process was done following the international guide Scientific Task Force on Forensic Toxicology. After sample extraction, derivatizing solution added, clean-up Hollow Fiber Liquid Phase Microextraction (HF-LPME) adapted use ultrasonic bath, simple easy-to-handle device. Isobutylchloroformate first used as derivatization reagent convert isobutylbenzoylecgonine. Analytes occurred within linear range 0.1 20 ng mg-1 COC 0.05 5 BZE CE, correlation coefficient r > 0.99. Limits were 0.05; 0.03 mg-1, whereas limits 0.1; COC; respectively. There no matrix effect interference. Intra inter-day precision accuracy acceptable according guidelines. analytical method HF-LPME-GC-MS successfully applied 14 samples from patients admitted drugs detoxification programs. All resulted positive (0.80-> mg-1) (0.20-> mg-1), 11 (0.10-> 0.60 mg-1).